Device

Part:BBa_K1421000

Designed by: Mengni Wang   Group: iGEM14_CAU_China   (2014-09-23)


plux R+RBS+LacIM1+2TE

This device is comprised of lux pR (BBa_R0062), RBS (BBa_B0034), lacIM1 (BBa_K082005) and 2 TE (BBa_B0010 and BBa_B0012). The lux pR is a right promoter in the lux cassette of V. Fischeri, which gives weak constitutive expression while enhanced when binding to the complex of LuxR and AHL (acyl-homoserine lactone ). The LacIM1 is a LacI mutant, which can repress lac promoter. Thanks to the work of Tokyo_Tech 2010, it is confirmed that this mutant protein has a weaker function of repression to the lac promoter, compared to the wild type LacI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None